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tripathi t advances protein molecular structural biology methods 2022

Type:: E-books
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Uploaded On:: Feb. 28, 2023, 10:10 a.m.
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Textbook in PDF format Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering.It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application. Strategies to improve the expression and solubility of recombinant proteins in E. coli Advances in heterologous protein expression strategies in yeast and insect systems Methods for transient expression and purification of monoclonal antibodies in mammalian cells Methods for recombinant production and purification of intrinsically disordered proteins Methods to determine the oligomeric structure of proteins Multimodal methods to study protein aggregation and fibrillation Experimental methods to study the thermodynamics of protein–protein interactions Experimental methods to study the kinetics of protein–protein interactions Computational techniques for studying protein-protein interactions Experimental methods to study protein–nucleic acid interactions Advanced computational tools for quantitative analysis of protein–nucleic acid interfaces Experimental techniques to study protein dynamics and conformations Computational techniques to study protein dynamics and conformations Application of circular dichroism spectroscopy in studying protein folding, stability, and interaction Studying protein-folding dynamics using single-molecule fluorescence methods Advances in liquid-state NMR spectroscopy to study the structure, function, and dynamics of biomacromolecules In-cell NMR spectroscopy: A tool to study cellular structure biology Current trends in membrane protein crystallography Advances in sample preparation and data processing for single-particle cryo-electron microscopy Advanced mass spectrometry-based methods for protein molecular-structural biologists

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Tripathi T. Advances in Protein Molecular and Structural Biology Methods 2022.pdf 134.8 MB

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tripathi t advances protein molecular structural biology methods 2022